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<p class="MsoNormal" style="background:white"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black">Forwarded on behalf of</span><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:#1F497D">:</span><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black">
<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black"><o:p> </o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black">Jules NeSmith, M.Sc.<br>
Interdisciplinary Ecology<br>
University of Florida<br>
<a href="http://www.florylab.com/" target="_blank"><span style="color:#1155CC">florylab.com</span></a><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-bottom:12.0pt;background:white"><span style="font-size:8.5pt;font-family:"Arial","sans-serif";color:#222222"><o:p> </o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:8.5pt;font-family:"Arial","sans-serif";color:#222222">Hi all, we need your help collecting Microstegium (stiltgrass) seed! We have a new grant where we are <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:8.5pt;font-family:"Arial","sans-serif";color:#222222">trying to understand the long-term effects of pathogens on invaded plant communities. Seed from <br>
populations throughout the invaded range would be helpful.<br>
<br>
Our seed collecting protocol is outlined below, note that we can cover shipping charges via FedEx. It <br>
would be helpful if you could provide a close up photo of the plants so we can determine if the <br>
population is infected, and if you happened to know the approximate age of the invasion that also could <br>
be useful.<br>
<br>
Please circulate this to others who might be able to help out.<br>
<br>
Thank you!<br>
<br>
<br>
__________________________________________________________________________________________________________<br>
Note: Microstegium seed is mature when it is mostly brown and can be easily shaken from the seed <br>
head. Seed collected while still green will have lower germination. Seed matures at the end of <br>
September through mid to late October depending on latitude, with more northern latitudes maturing <br>
first.<br>
<br>
Microstegium has a mixed mating system, which means that it produces seed by both selfing and <br>
outcrossing. Outcrossed or chasmogomous seed is the seed that you see produced at the end of tillers <br>
and those are the seeds we are interested in obtaining.<br>
<br>
We are focused on among population variation, not within population, so to collect seed you can simply <br>
collect seed from anywhere within an approximately 20m diameter area. The simplest method is to <br>
arbitrarily walk around in a given area, grab the ends of tillers in large bunches, and shake the seed <br>
heads into a paper grocery bag.<br>
<br>
We need about at least 50ml or ¼ cup of seed from each population for it to be useful. That amount can <br>
be collected from with a dense invasion in about 5-10 minutes. Collecting more seed could be very <br>
helpful.<br>
<br>
We need the approximate lat/long coordinates of the collection location. If you happen to be in the field <br>
a lot and can collect seed from multiple populations, they need to be separated by 2-5 km. A short <br>
description of the site would also be useful.<br>
<br>
Please send seed in a padded envelope to:<br>
<br>
S. Luke Flory<br>
Agronomy Dept, IFAS<br>
McCarty B 3127<br>
Gainesville, FL, 32611<br>
<br>
Cell: <a href="tel:(352)%20231-2376" target="_blank"><span style="color:#1155CC">352-231-2376</span></a><br>
Email: <a href="mailto:flory@ufl.edu" target="_blank"><span style="color:#1155CC">flory@ufl.edu</span></a><br>
<br>
* For international shipments contact me via email prior to shipping! I will need to provide a permit and <br>
special shipping label. There are specific packing instructions.<br>
<br>
If you can ship FedEx I can provide you with an account number for billing.<br>
<br>
Thanks!<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-size:8.5pt;font-family:"Arial","sans-serif";color:#222222">Jules NeSmith (on behalf of) S. Luke Flory<br>
University of Florida</span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">_________________________________________________________________________<o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt">Damien P. Ossi<o:p></o:p></span></b></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Wildlife Biologist<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Fisheries and Wildlife Division<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Department of Energy & Environment<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Government of the District of Columbia<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">1200 First Street NE, 5th Floor<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Washington, DC 20002<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Desk: (202) 741-0840<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Web: <a href="http://www.doee.dc.gov/">
doee.dc.gov</a><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p>The new school year is here and every day is a new opportunity for our students to learn and grow. We all have a role to play in ensuring students get to school, ready to learn, and understand that #EveryDayCounts. Go to
<a href="http://attendance.dc.gov"><span style="color:#0000ff;"><u>attendance.dc.gov</u></span></a> to learn more.</p>
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