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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'>Hi. Who can help Carrie?<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'>Marc<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b>From:</b> Wu, Carrie <cwu@richmond.edu> <br><b>Sent:</b> Monday, July 20, 2020 11:12 AM<br><b>To:</b> Marc Imlay <ialm@erols.com><br><b>Subject:</b> need wavyleaf tissue / seeds again, please!<o:p></o:p></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal>Hi Marc, <o:p></o:p></p><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>I hope all is as well with you as can be expected during these tumultuous times.<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>As most of my lab work has been on pause due to covid-19, I’m focusing efforts on increasing my sampling of wavyleaf basketgrass across the invaded range this summer. Would you be willing to share this request with the MAIPC listserv? I had sent a smiler email to a few of you who helped me out earlier, too.<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>Thanks,<o:p></o:p></p></div><div><p class=MsoNormal>Carrie<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>*********<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>Hi all,<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>I’ve been working on a project over the past few years to understand the spatial genetic structure of wavy leaf basketgrass, and spread across the landscape. With our previous collections (many provided by folks on this listserv), my lab group was able to develop the first set of genetic markers for wavyleaf and confirm the population in Patapsco Valley State Park in MD as the likely source, with multiple routes of dispersal from that initial site. We’re now poised to conduct a more comprehensive analysis across the known invasion range, which has expanded from a few years ago.<o:p></o:p></p></div><div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>This summer, I’m seeking additional collections from as many locations as possible across the introduced range. Would you be able to help me out again? The goal is to have 10-15 individual plants collected from large populations when possible (with individual plants at least 3 feet apart from one another). Smaller populations would have a reduced number of plants sampled. Observations from several folks suggest that wavyleaf seems to be setting seed earlier than several years ago, so if you encounter seeds later this summer, those would be greatly appreciated too.<o:p></o:p></p></div></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><div><p class=MsoNormal>Tissue sampling is pretty straight forward, especially if the plants aren’t too wet. I can send a detailed protocol if requested. In brief,<o:p></o:p></p></div></div><ul type=disc><li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1'>Record collector and site information, including GPS coordinates<o:p></o:p></li><li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1'>Collect at least 5-8 fully expanded leaves (or entire stalks!) per plant into coin envelopes/regular envelopes/paper bags. <b>Please keep leaves from each individual plant in separate bags/wrappings</b><o:p></o:p></li><li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1'>If storing for an extended time, place filled envelopes in a plastic bag with a little silica drying gel (or the “Do Not Eat” packets that come in lots of items)<o:p></o:p></li><li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1'>When sampling multiple plants in population, try to separate collections by at least 1 meter<o:p></o:p></li><li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1'>Mail them to me! I am happy to offset shipping costs as needed.<o:p></o:p></li></ul><div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>If you are able to collect tissue, or would be willing to have me access sites where you know the plants are growing, please let me know! I can provide more detailed sampling instructions as needed. <o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>And of course, please share this request widely with colleagues who you think might also be able to help!<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>Many thanks,<o:p></o:p></p></div><div><p class=MsoNormal>Carrie<o:p></o:p></p></div></div></div><div><div><div><div><div><div><p class=MsoNormal style='margin-bottom:12.0pt'><span style='color:black'>____________________________________________<br>Carrie Wu, Ph.D.<br>Associate Professor of Biology<br>Coordinator, Environmental Studies Program<br>University of Richmond<br>138 UR Drive<br>Richmond, VA 23173<br><br>Office: A114 Gottwald Science Center<br>Phone: (804) 289-8712<o:p></o:p></span></p></div></div></div><p class=MsoNormal><o:p> </o:p></p></div></div></div></div><div id="DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2">
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