<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><head><meta http-equiv=Content-Type content="text/html; charset=us-ascii"><meta name=Generator content="Microsoft Word 15 (filtered medium)"><!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:Helvetica;
panose-1:2 11 6 4 2 2 2 2 2 4;}
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:"Trebuchet MS";
panose-1:2 11 6 3 2 2 2 2 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:#0563C1;
text-decoration:underline;}
p.xmsonormal, li.xmsonormal, div.xmsonormal
{mso-style-name:x_msonormal;
margin:0in;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
span.EmailStyle22
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'>Matt Tancos and his team are doing a great job looking for pathogen biocontrols. Who is looking for insect biocontrols in a similar way?<o:p></o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b>From:</b> Tancos, Matthew - ARS <<a href="mailto:matthew.tancos@usda.gov">matthew.tancos@usda.gov</a>> <br><b>Sent:</b> Friday, June 11, 2021 8:18 AM<br><b>To:</b> <a href="mailto:ialm@erols.com">ialm@erols.com</a><br><b>Subject:</b> Re: [External Email]biological control of wineberry<o:p></o:p></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal><span style='font-size:12.0pt;color:black'>Hi Marc,<o:p></o:p></span></p></div><div><p class=MsoNormal><span style='font-size:12.0pt;color:black'>No, we were not planning on pursuing this further after Bill's initial work. However, I will speak with Bill and see if this pathogen has had any impact on this specific infestation over the years. Also,<o:p></o:p></span></p></div><div><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif;color:red'>I was able to identify a putative pathogen of fig buttercup this year. We are currently investigating the microorganisms and trying to get healthy fig buttercup for inoculations to verify pathogenicity. Hopefully, we find something interesting!<o:p></o:p></span></p></div><div><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif;color:red'><o:p> </o:p></span></p></div><div><p class=MsoNormal><span style='font-size:12.0pt;color:black'>best,<o:p></o:p></span></p></div><div><p class=MsoNormal><span style='font-size:12.0pt;color:black'>Matt <o:p></o:p></span></p></div><div><div><p class=MsoNormal><span style='font-size:12.0pt;color:black'><o:p> </o:p></span></p></div><div id=Signature><div><div><p style='background:white'><span style='color:#201F1E'>Matthew A. Tancos, PhD.<o:p></o:p></span></p><p style='background:white;text-align:start'><span style='color:#201F1E'>Research Plant Pathologist<o:p></o:p></span></p><p style='background:white;text-align:start'><span style='color:#201F1E'>USDA Agricultural Research Service<o:p></o:p></span></p><p style='background:white;text-align:start'><span style='color:#201F1E'>Foreign Disease-Weed Science Research Unit<o:p></o:p></span></p><p style='background:white;text-align:start'><span style='color:#201F1E'>1301 Ditto Avenue<o:p></o:p></span></p><p style='background:white;text-align:start'><span style='color:#201F1E'>Fort Detrick, MD 21702<o:p></o:p></span></p><p style='background:white;text-align:start'><span style='color:#201F1E'>Tel: 301-619-7386<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:12.0pt;color:black'><o:p> </o:p></span></p></div></div></div></div><div class=MsoNormal align=center style='text-align:center'><hr size=2 width="98%" align=center></div><div id=divRplyFwdMsg><p class=MsoNormal><b><span style='color:black'>From:</span></b><span style='color:black'> Marc Imlay <<a href="mailto:ialm@erols.com">ialm@erols.com</a>><br><b>Sent:</b> Thursday, June 10, 2021 4:38 PM<br><b>To:</b> Tancos, Matthew - ARS <<a href="mailto:matthew.tancos@usda.gov">matthew.tancos@usda.gov</a>><br><b>Subject:</b> [External Email]biological control of wineberry</span> <o:p></o:p></p><div><p class=MsoNormal> <o:p></o:p></p></div></div><div><div style='border:solid greenyellow 1.5pt;padding:1.0pt 1.0pt 1.0pt 1.0pt;margin-left:1.5pt;margin-top:1.5pt;margin-right:1.5pt;margin-bottom:1.5pt'><p class=MsoNormal style='background:ghostwhite'><span style='font-size:9.0pt;color:black;background:greenyellow'>[External Email]</span><span style='font-size:9.0pt;color:black'> <br>If this message comes from an <b>unexpected sender</b> or references a <b>vague/unexpected topic;</b> <br>Use caution before clicking links or opening attachments.<br>Please send any concerns or suspicious messages to: <a href="mailto:Spam.Abuse@usda.gov">Spam.Abuse@usda.gov</a> </span><span style='font-size:9.0pt'><o:p></o:p></span></p></div><div><div><p class=xmsonormal> <o:p></o:p></p><p class=xmsonormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'>So does anyone plan to go to Frederick MD to see if the new biocontrol of wineberry is still there?</span><o:p></o:p></p><p class=xmsonormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'> </span><o:p></o:p></p><p class=xmsonormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'>Marc</span><o:p></o:p></p><p class=xmsonormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'> </span><o:p></o:p></p><p class=xmsonormal><span style='font-size:14.0pt;font-family:"Arial",sans-serif'> </span><o:p></o:p></p><table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width="100%" style='width:100.0%;background:white;border-collapse:collapse'><tr><td valign=top style='padding:0in 0in 0in 0in'><p class=xmsonormal style='margin-bottom:13.0pt'><span style='font-size:13.0pt;font-family:"Helvetica",sans-serif;color:#333333'>Bruckart, W.L., Eskandari, F. 2015. First Report of a Leaf Spot caused by Sphaerulina tirolensis on Rubus phoenicolasius. Plant Disease. Volume 99, Number 9, Page 1275.</span><o:p></o:p></p><p class=xmsonormal style='margin-bottom:12.0pt'><b><span style='font-size:13.0pt;font-family:"Helvetica",sans-serif;color:#333333'>Technical Abstract:</span></b><span style='font-size:13.0pt;font-family:"Helvetica",sans-serif;color:#333333'> Diseased leaves of Rubus phoenicolasius (wineberry) were discovered on October 11, 2013 in a small, 3x3 m, infestation at a sunny location along Indian Springs Rd., Frederick, MD (N 39.467634, W 77.461362). Although the proportion of diseased plants was estimated to be less than10 per cent of the population, several leaves had numerous, angular, discrete necrotic spots, as large as 1 mm diam., characterized by white or light gray, necrotic centers containing pycnidia and distinct, thin, dark red margins. Isolation from surface-sterilized leaf tissue on acidified potato dextrose agar resulted in recovery of a Septoria-like fungus (FDWSRU 13-028). Koch’s postulates were satisfied after two separate inoculations of five healthy cut canes each of R. phoenicolasius from greenhouse-propagated plants; the same fungus was reisolated after each inoculation. The isolate was characterized by narrow, tubular, long, hyaline, and straight, curved or flexuous conidia having 4–6(–8) septations and measuring (mean [c.i.], P = 0.05; w x l) 2.25 [0.12] x 54.5 [ 4.6] microns. DNA was extracted from the isolate using DNEasy and sequenced for ITS1, 5.8S, and ITS2 (GenBank No. KM408152). Using the BLAST option in GenBank, two accessions from raspberry, Rubus idaeus (GenBank Nos. KF251638 and KF251637), listed as “living cultures ex-type CBS 109017, 109018” by Verkley et al (2013), were 99 per cent similar, having only one nucleotide difference from the FDWSRU 13-028 sequence. The pathogen is tentatively identified as Sphaerulina cf. tirolensis Verkley, Quaedvlieg & Crous, on the basis of similar conidium morphological (Ellis et al. 1991, Verkley et al. 2013) and molecular characteristics (Verkley et al. 2013). Specimens have been submitted to the USDA, ARS, SMML and to the CBS Fungal Biodiversity Centre in Utrecht, The Netherlands. Wineberry is a raspberry that was introduced from Asia and is now an invasive species in the eastern USA. This is the first report of S. tirolensis, a raspberry pathogen, on R. phoenicolasius. Characterization of diseases at new locations for plant introductions is fundamental to understanding ecological events and interactions where the host is introduced. This disease is likely established in the USA, because symptomatic plants were found at a new site near the original patch on August 15, 2014, and observations of significant increase in incidence have been made in September, 2014. At the present level of disease and the fact that symptoms appear after fruiting, it seems unlikely that this leaf spot is affecting population density to any significant level.</span><o:p></o:p></p></td></tr></table><p class=xmsonormal> <o:p></o:p></p><p class=xmsonormal><a href="https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ars.usda.gov%2Fresearch%2Fpublications%2Fpublication%2F%3FseqNo115%3D310070&data=04%7C01%7C%7Ce041758f616d4c9d340308d92c4fab95%7Ced5b36e701ee4ebc867ee03cfa0d4697%7C0%7C0%7C637589542958586630%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gRcgoNf1AQi6WZesJWQiZi6ZMXNG1Xjpozlffx8YOAc%3D&reserved=0">https://www.ars.usda.gov/research/publications/publication/?seqNo115=310070</a><o:p></o:p></p><p class=xmsonormal><b><span style='font-size:13.0pt;font-family:"Helvetica",sans-serif;color:#333333;background:white'>Author</span></b><o:p></o:p></p><table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 style='border-collapse:collapse'><tr><td valign=top style='background:white;padding:2.25pt 2.25pt 2.25pt 2.25pt'><p class=xmsonormal><span style='color:black'><img border=0 width=16 height=18 style='width:.1666in;height:.1875in' id="x_Picture_x0020_1" src="cid:image001.gif@01D6D240.B726C6C0" alt=item></span><o:p></o:p></p></td><td valign=top style='background:white;padding:2.25pt 2.25pt 2.25pt 2.25pt'><p class=xmsonormal><span style='color:black'><a href="https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ars.usda.gov%2Fpeople-locations%2Fperson%3Fperson-id%3D698&data=04%7C01%7C%7Ce041758f616d4c9d340308d92c4fab95%7Ced5b36e701ee4ebc867ee03cfa0d4697%7C0%7C0%7C637589542958586630%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=dn90OrG0VHJE%2FGFDgQo6qo87gO%2FW52E4lj4DoE7gcoA%3D&reserved=0"><span style='color:#4C2C92'>Bruckart, William - <i>Bill</i></span></a></span><o:p></o:p></p></td></tr></table><p class=xmsonormal> <o:p></o:p></p><p class=xmsonormal><span style='font-size:10.5pt;font-family:"Trebuchet MS",sans-serif;color:#333333'>Biological control: Numerous diseases and insects affect wineberry, including wineberry latent virus. See Ellis and others [</span><a href="https://gcc02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.fs.fed.us%2Fdatabase%2Ffeis%2Fplants%2Fshrub%2Frubpho%2Fall.html%2317&data=04%7C01%7C%7Ce041758f616d4c9d340308d92c4fab95%7Ced5b36e701ee4ebc867ee03cfa0d4697%7C0%7C0%7C637589542958596707%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=AaS6dpr4MKIDmFmmXrzOJLErGQ%2BH4KeAqLih8K0ZG6w%3D&reserved=0"><span style='font-size:10.5pt;font-family:"Trebuchet MS",sans-serif;color:#337AB7'>17</span></a><span style='font-size:10.5pt;font-family:"Trebuchet MS",sans-serif;color:#333333'>] for a review.</span><o:p></o:p></p><p class=xmsonormal><a href="https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.inaturalist.org%2Fguide_taxa%2F354717&data=04%7C01%7C%7Ce041758f616d4c9d340308d92c4fab95%7Ced5b36e701ee4ebc867ee03cfa0d4697%7C0%7C0%7C637589542958596707%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=I91NRoKlD7YxmDvZt9aa3DWg7bj1P40BkcEAdHNTlSs%3D&reserved=0">https://www.inaturalist.org/guide_taxa/354717</a><o:p></o:p></p><p class=xmsonormal><a name="x_17"><span style='font-size:13.5pt;font-family:"Times New Roman",serif;color:black;background:white'>17.</span></a><span style='font-size:13.5pt;font-family:"Times New Roman",serif;color:black;background:white'> Ellis, Michael A.; Converse, Richard H.; Williams, Roger N.; Williamson, Brian. 1997. Compendium of raspberry and blackberry diseases and insects. St. Paul, MN: The American Phytopathological Society. 100 p. [73168]</span><o:p></o:p></p><div id="x_DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2"><p class=xmsonormal style='margin-bottom:12.0pt'> <o:p></o:p></p><p class=xmsonormal style='margin-bottom:12.0pt'> <o:p></o:p></p></div></div></div></div><p class=MsoNormal><br><br><br><br>This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. <o:p></o:p></p><p class=MsoNormal>You received this email because you are subscribed to the Maryland Invasive Species Council (INVASIVES) mailing list. If you would like to unsubscribe from this list, simply send an email to <a href="mailto:listserv@listserv.umd.edu">listserv@listserv.umd.edu</a> with the message signoff INVASIVES in the body. <o:p></o:p></p></div></body></html>