[MAIPC] [EXTERNAL] wavyleaf seeds / leaf tissue request again

[Spencer, Sandy]

Hi Carrie,
We have an infestation of WLBG on the Patuxent refuge.   I will try to collect leaf and seed samples for you. Thanks for including the protocol.

Sandy Spencer
Supervisory Refuge Biologist
Patuxent Research Refuge
U.S. Fish and Wildlife Service
12100  Beech Forest Road, Suite 138
Laurel, MD  20708
301.497.5587 (ofc)
240.882.1077 (cell)
sandy_spencer at fws.gov

________________________________
From: MAIPC <maipc-bounces at lists.maipc.org> on behalf of Wu, Carrie <cwu at richmond.edu>
Sent: Wednesday, September 8, 2021 8:35 AM
To: maipc at lists.maipc.org <maipc at lists.maipc.org>
Subject: [EXTERNAL] [MAIPC] wavyleaf seeds / leaf tissue request again




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Hello all,

This is my semi-regular request for seed and/or leaf tissue samples of wavyleaf basket grass from across the known invasion range. At this time of year, I’m especially looking for ripe seed collections.

We’ve developed a protocol that allows us to grow plants from seed in secured growth chambers, and have begun to examine germination and growth responses under varied environmental conditions.  So far we have seeds collected from a small number of populations, and now are looking to expand this.

Tissue sampling is pretty straight forward, especially if the plants aren’t too wet.  I can send a detailed protocol if requested.  In brief,

  *   Record collector and site information, including GPS coordinates
  *   Collect at least 3-4 leaves (or entire stalks!) per plant into coin envelopes/regular envelopes/paper bags
  *   Seeds can be pooled from several plants. If doing so, please indicate that the seeds are a population collection, rather than from a single plant.
  *   If storing for an extended time, place filled envelopes in a plastic bag with a little silica drying gel (or the “Do Not Eat” packets)
  *   When sampling for leaves from multiple plants per population, separate collections by at least 1 meter
  *   Mail them to me!

Many thanks to those who have already sent me samples; these are archived on campus and my research team is making steady progress with genetic analyses.

And of course, please share this request widely with colleagues who you think may also be able to help.  I am happy to offset collection/shipping costs as needed.

Best,
Carrie
____________________________________________
Carrie Wu, Ph.D.
Associate Professor of Biology
Coordinator, Environmental Studies Program
University of Richmond
138 UR Drive
Richmond, VA 23173

Office: A114 Gottwald Science Center
Phone: (804) 289-8712


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